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Arnold, Dawn and Neale, Helen (2020) An improved conjugation method for Pseudomonas syringae. UWE https://researchdata.uwe.ac.uk/id/eprint/583/
Brief summary of project
In order achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
| UWE College/School: | College of Health, Science and Society > School of Applied Sciences |
|---|---|
| Creators: | Arnold, Dawn and Neale, Helen |
| URI: | https://researchdata.uwe.ac.uk/id/eprint/583 |
| Data collection method: | Improved version of previous conjugation method |
| Resource language: | English |
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- Arnold, Dawn
- Neale, Helen
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