An improved conjugation method for Pseudomonas syringae

How to cite this record

Arnold, D. and Neale, H. (2020) An improved conjugation method for Pseudomonas syringae. UWE, http://researchdata.uwe.ac.uk/583.

UWE Harvard citation (for UWE users)

Arnold, D. and Neale, H. (2020) An improved conjugation method for Pseudomonas syringae. UWE data repository [online]. Available from: http://researchdata.uwe.ac.uk/583 [Accessed 29 August 2020].

Project Title

An improved conjugation method for Pseudomonas syringae

Brief summary of project

In order achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

Associated Publication Links

https://uwe-repository.worktribe.com/output/6508170 (UWE Research Repository)

Publisher

UWE

Details

Item Type: Dataset
Methodology: Improved version of previous conjugation method
Language of the Dataset Collection: English
Creators: Arnold, D. and Neale, H.
UWE Faculty/Department: Faculty of Health and Applied Sciences > Department of Applied Sciences
UWE Research Centres/Institutes: Faculty of Health and Applied Sciences > Centre for Research in Biosciences
Depositing User: Professor D. Arnold
Date Deposited: 26 Aug 2020 09:46
Last Modified: 26 Aug 2020 09:46

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