Brenneria goodwinii growth in vitro is improved by competitive interactions with other bacterial species associated with Acute Oak Decline

How to cite this record

Brady, C. and Orsi, M. (2021) Brenneria goodwinii growth in vitro is improved by competitive interactions with other bacterial species associated with Acute Oak Decline. UWE, http://researchdata.uwe.ac.uk/633.

UWE Harvard citation (for UWE users)

Brady, C. and Orsi, M. (2021) Brenneria goodwinii growth in vitro is improved by competitive interactions with other bacterial species associated with Acute Oak Decline. UWE data repository [online]. Available from: http://researchdata.uwe.ac.uk/633 [Accessed 22 September 2021].

Project Title

Brenneria goodwinii growth in vitro is improved by competitive interactions with other bacterial species associated with Acute Oak Decline

Brief summary of project

Brenneria goodwinii, Rahnella victoriana and Gibbsiella quercinecans are three bacterial species frequently isolated together from oak displaying symptoms of Acute Oak Decline (AOD), which include weeping patches on trunks. All three bacterial species play a role in lesion formation in the current episode of AOD in Britain, although B. goodwinii is the most dominant. The ongoing research into stem lesion formation characteristic of this polybacterial syndrome has been focussed primarily on the pathogenicity, identification and taxonomy of these bacteria. As all three species were newly classified within the past ten years, there are many unanswered questions regarding their ecology and interactions with each other. To determine the effect of bacterial interactions on fitness in vitro, we examined pairwise (diculture) and multispecies (triculture) interactions between B. goodwinii, R. victoriana and G. quercinecans in oak leaf media microcosms, and also determined the effect of co-culturing on the evolution of these species. Our results indicate that B. goodwinii thrived in monoculture with significantly higher viable cell counts than the other two species. Additionally, B. goodwinii performed well in pairwise culture with mutually competitive interactions observed between B. goodwinii and R. victoriana, and between B. goodwinii and G. quercinecans. In the multispecies triculture, B. goodwinii and R. victoriana appeared to exhibit co-ordinated behaviour to outcompete G. quercinecans. After four weeks B. goodwinii grown in co-culture with the other two species developed greater evolved fitness than the strain grown in monoculture as reflected by the increased viable cell counts. The competitive interactions taking place between the threes species indicated evolving improved fitness of B. goodwinii in vitro, that gave it a growth advantage over both R. victoriana and G. quercinecans which showed no significant changes in fitness. Overall, B. goodwinii gains greater benefit in terms of fitness from in vitro competitive interaction with the other two species.

Publisher

UWE

Details

Item Type: Dataset
Methodology: General linear models were used to compare bacterial growth across microcosms. For the microcosm growth experiments, the response variable was either the biomass (measured by optical density) or the log10 of the viable colony counts. The factors considered were time (weeks 1 to 4), microcosm composition (mono-, di- and tri-cultures), and their interaction. For the growth assay of evolved species experiment, the response variable was the log10 of the viable colony counts for each of the three constituent species and the factors considered were the strain inoculum origins (ancestral strains and re-isolated strains from microcosms). Post hoc testing was conducted using the Bonferroni pairwise comparisons method (95 % confidence level). Calculations were done in Minitab 17 (Minitab 17 Statistical Software, 2010).
Language of the Dataset Collection: English
Creators: Brady, C. and Orsi, M.
UWE Faculty/Department: Faculty of Health and Applied Sciences > Department of Applied Sciences
UWE Research Centres/Institutes: Faculty of Health and Applied Sciences > Centre for Research in Biosciences
Depositing User: Dr C. Brady
Date Deposited: 20 Sep 2021 08:12
Last Modified: 20 Sep 2021 08:12

Data files

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MicrocosmBiomass_GLM_DetailedComparisons.mpx
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Microcosm-LogCount_GLM-comparisons.mpx
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GrowthRate_Bg_Log_detailed.mpx
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GrowthRate_Gq_Log.mpx
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GrowthRate_Rvic_Log_detailed.mpx
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