Transposon mutagenesis of Pseudomonas syringae pvs. syringae and morsprunorum to identify genes required to cause bacterial canker disease of cherry.

How to cite this record

Neale, H. and Arnold, D. and Jackson, R. and Hulin, M. and Harrison, R. (2021) Transposon mutagenesis of Pseudomonas syringae pvs. syringae and morsprunorum to identify genes required to cause bacterial canker disease of cherry. UWE, http://researchdata.uwe.ac.uk/627.

UWE Harvard citation (for UWE users)

Neale, H. and Arnold, D. and Jackson, R. and Hulin, M. and Harrison, R. (2021) Transposon mutagenesis of Pseudomonas syringae pvs. syringae and morsprunorum to identify genes required to cause bacterial canker disease of cherry. UWE data repository [online]. Available from: http://researchdata.uwe.ac.uk/627 [Accessed 20 May 2021].

Project Title

Transposon mutagenesis of Pseudomonas syringae pvs. syringae and morsprunorum to identify genes required to cause bacterial canker disease of cherry.

Brief summary of project

Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syrinage (P.s.). Strains from two pathovars P.s. pv. syringae (Pss) and P.s. pv. morsprunorum race 1 (PsmR1) and 2 (PsmR2) in three phylogenetically distant clades have convergently evolved to infect Prunus. The bacteria enter woody tissues through wounds and leaf scars causing black necrotic cankers. Symptoms are also produced on blossom, fruit and leaves. Little is known about the mechanisms P.s. uses to colonise tree hosts such as Prunus. Here we created transposon (Tn) mutant libraries in one strain of P.s. from each of the three clades and screened the mutants on immature cherry fruit to look for changes in virulence. Mutants (242) with either reduced or enhanced virulence were detected and further characterised by in vitro screens for biofilm formation, swarming ability, and pathogenicity on leaves and cut shoots. In total 18 genes affecting virulence were selected, and these were involved in diverse functions including motility, Type III secretion, membrane transport, amino acid synthesis, DNA repair and primary metabolism. Interestingly, mutation of the effector gene hopAU1, led to an increase in virulence in Psm R2.

Publisher

UWE

Details

Item Type: Dataset
Methodology: Plant phenotypic assays including motility, growth in vitro and in planta, attachments assays, and pathogenicity test. Sequencing.
Language of the Dataset Collection: English
Creators: Neale, H. and Arnold, D. and Jackson, R. and Hulin, M. and Harrison, R.
UWE Faculty/Department: Faculty of Health and Applied Sciences
UWE Research Centres/Institutes: Faculty of Health and Applied Sciences > Centre for Research in Biosciences
Depositing User: Dr H. Neale
Date Deposited: 20 May 2021 07:20
Last Modified: 20 May 2021 07:20

Data files

[img] Spreadsheet
Tn paper raw data file.xlsx
Available under License Creative Commons Attribution Share Alike.

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